Human Anti-Mullerian Hormone CLIA Kit

Description

This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of human Anti-Müllerian Hormone (AMH) levels in serum or plasma using the ECL100 or ECL-25 Immunoassay analyzer.               

It is for in-vitro diagnostics use only.

Background

This CLIA is designed, developed, and produced for the quantitative measurement of human AMH level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of AMH.

​​​​​​​Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Simultaneously, anacridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles– biotin AMH antibody –AMH–acridinium ester AMH antibody”.
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The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL-25 analyzer. The relative light units (RLU) are proportional to the concentration of AMH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum AMH concentration.

Selected Literature

1. Karkanaki, A., Vosnakis, C., &Panidis, D. The clinical significance of anti-Müllerian hormone evaluation in gynecological endocrinology. Hormones, 2011; 10(2):95–103. 
2. Hampl, R., Šnajderová, M., &Mardešić, T.  Antimüllerian Hormone (AMH) Not Only a Marker for Prediction of Ovarian Reserve. Physiological Research, 2011; 10:217–223. 

For in-vitro diagnostic use.