Description

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Background

The Human Prolactin CLIA Kit is designed, developed, and produced for the quantitative measurement of human PRL level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of PRL.
​​​​​​​Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the PRL in the form of “magnetic particles–PRL antibody–PRL–acridinium ester PRL antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a PRL in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum PRL concentration.

Selected Literature

​​​​​​​1.Tarquini B,Gheri R, NeriB, et al. Circadian study of immunoreactive prolactin in patients with cirrhosis of the liver.Gastroenterology, 1977, 73(1): 116-119.
2. KarimanN, Hedayati M, Majd SA. The diagnostic power of cervico-vaginal fluid prolactin in the diagnosis of premature rupture of membranes.Iran Red Crescent Med, 2012, 14(9): 541-548.
​​​​​​​3. Keeler C, TettamanziMC, MeshackS, et al. Contribution of individual histidines to the global stability of human prolactin. Protein Sci, 2009, 18: 909-920.

For in-vitro diagnostic use.