Description

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Background

The Human Myoglobin CLIA Kit is designed, developed, and produced for the quantitative measurement of human Myoglobin （MYO） level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of MYO. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the MYO in the form of “magnetic particles–MYO antibody–MYO– acridinium ester MYO antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a MYO in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum MYO concentration.
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Selected Literature

​​​​​​​1.Chapelle JP, Lemache K, EI Allaf M, et al. Fast Determination of Myoglobin in serum Using a New Radial Partition Immunoassay. Clinical Biochemistry, 1994 ,27: 423-8.
2.Kagen L, Scheidt S, Roberts L, et al. Myoglobinemia following myocardial infarction. Am J Med, 1975,58: 177-182.
​​​​​​​3.Nieuwenhoven FA. Discrimination between myocardial and skeletal muscle injury by assessment of the plasma ratio of myoglobin over fatty acid-binding protein. Circulation, 1995, 92(10): 2848-2854.

For in-vitro diagnostic use.